Q5. Which Of the following is/are NOT the step(s) used in separation and isolation of DNA fragments

Q: What is the correct answer for CUET UG Biology Q5?

The correct answer is A. The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. The bright orange coloured bands of DNA in a ethidium bromide stained gel can be seen w

Q: Q5. Which Of the following is/are NOT the step(s) used in separation and isolation of DNA fragments by gel electrophoresis technique: (A) Staining with ethedium bromide (B) Exposure to UV…

The correct answer is A. The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. The bright orange coloured bands of DNA in a ethidium bromide stained gel can be seen w

Q: What are the options for this CUET UG Biology question?

A. (D) only · B. (B) and (C) only · C. (B) only · D. (A) and (D) only

Question

Q5. Which Of the following is/are NOT the step(s) used in separation and isolation of DNA fragments by gel electrophoresis technique:
(A) Staining with ethedium bromide
(B) Exposure to UV Radiation
(C) Cutting DNA fragments into small pieces by using restriction endonuclease
(D) Denaturation of DNA fragments
Choose the correct answer from the options given below:

FirstInTest · Accepted Answer

The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. The bright orange coloured bands of DNA in a ethidium bromide stained gel can be seen when exposed to UV light.Steps involved in gel electrophoresis for separation and isolation:1. Cutting DNA fragments using restriction endonuclease (C).2. Separation of DNA fragments by gel electrophoresis.3. Staining with ethidium bromide (A).4. Exposure to UV radiation (B) for visualization.Denaturation of DNA fragments is not a standard step in the separation and isolation of DNA fragments by gel electrophoresis.

Answer

(D) only

Answer

(B) and (C) only

Answer

(B) only

Answer

(A) and (D) only

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