Q29. Arrange the following steps of rDNA technology in correct sequence. (A) Amplification of gene b

Q: What is the correct answer for CUET UG Biology Q29?

The correct answer is a. DNA containing the gene of interest is identified and isolated from the organism. The gene of interest is inserted into the plasmid which is a vector which is used to deliver and maintain the DNA in the host organism. This is known as recombinant DNA or rDNA. The rDNA is now introduced into the host and the process is known as transformation. The host cells that have taken up the rDNA are known as

Q: Q29. Arrange the following steps of rDNA technology in correct sequence. (A) Amplification of gene by PCR (B) Insertion of rDNA into host cell using vector (C) Isolation of the genetic material from…

The correct answer is a. DNA containing the gene of interest is identified and isolated from the organism. The gene of interest is inserted into the plasmid which is a vector which is used to deliver and maintain the DNA in the host organism. This is known as recombinant DNA or rDNA. The rDNA is now introduced into the host and the process is known as transformation. The host cells that have taken up the rDNA are known as

Q: What are the options for this CUET UG Biology question?

A. (D), (C), (B), (A) · B. (C), (D), (B), (A) · C. (D), (C), (A), (B) · D. (A), (C), (D), (B)

Question

Q29. Arrange the following steps of rDNA technology in correct sequence. (A) Amplification of gene by PCR (B) Insertion of rDNA into host cell using vector (C) Isolation of the genetic material from the cell (D) Cutting the DNA at specific location Choose the correct answer from the options given below:

FirstInTest · Accepted Answer

DNA containing the gene of interest is identified and isolated from the organism. The gene of interest is inserted into the plasmid which is a vector which is used to deliver and maintain the DNA in the host organism. This is known as recombinant DNA or rDNA. The rDNA is now introduced into the host and the process is known as transformation. The host cells that have taken up the rDNA are known as transformed cells. These cells need to be differentiated from the other cells that have not taken up the rDNA. Here, growing the mixture of cells in an antibiotic-containing medium helps in the selection and identification of the transformants from the non-transformants. The various selectable markers also play an important role in identifying the recombinants from the non-recombinants. The rDNA containing the gene of interest expresses itself by producing a protein and this is known as the expression of the introduced gene.

Answer

(D), (C), (B), (A)

Answer

(C), (D), (B), (A)

Answer

(D), (C), (A), (B)

Answer

(A), (C), (D), (B)

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