Q14. Arrange the steps of gel electrophoresis in a proper
sequence:
(A) Fragments of DNA are separat

Q: What is the correct answer for CUET UG Biology Q14?

The correct answer is c. Gel electrophoresis is a technique used to separate DNA fragments based on their size and charge. The correct sequence of steps is as follows: 1. **(A) Fragments of DNA are separated under an electric field in agarose gel:** DNA samples are loaded into wells in an agarose gel, and an electric current is applied. Due to their negative charge, DNA fragments migrate towards the positive electrode, w

Q: Q14. Arrange the steps of gel electrophoresis in a proper sequence: (A) Fragments of DNA are separated under an electric field in agarose gel (B) DNA bands are cut out from the agarose gel…

The correct answer is c. Gel electrophoresis is a technique used to separate DNA fragments based on their size and charge. The correct sequence of steps is as follows: 1. **(A) Fragments of DNA are separated under an electric field in agarose gel:** DNA samples are loaded into wells in an agarose gel, and an electric current is applied. Due to their negative charge, DNA fragments migrate towards the positive electrode, w

Q: What are the options for this CUET UG Biology question?

A. (A), (C), (D), (B) · B. (C), (B), (A), (D) · C. (A), (D), (C), (B) · D. (B), (A), (D), (C)

Question

Q14. Arrange the steps of gel electrophoresis in a proper
sequence:
(A) Fragments of DNA are separated under an
electric field in agarose gel
(B) DNA bands are cut out from the agarose gel and
extracted
(C) DNA fragments are visualized by exposure to UV
radiation
(D) Separated DNA fragment stained with ethidium
bromide
Choose the correct answer from the options given
below:
(a) (A), (C), (D), (B)
(b) (C), (B), (A), (D)
(c) (A), (D), (C), (B)
(d) (B), (A), (D), (C)

FirstInTest · Accepted Answer

Gel electrophoresis is a technique used to separate DNA fragments based on their size and charge. The correct sequence of steps is as follows:
1.  **(A) Fragments of DNA are separated under an electric field in agarose gel:** DNA samples are loaded into wells in an agarose gel, and an electric current is applied. Due to their negative charge, DNA fragments migrate towards the positive electrode, with smaller fragments moving faster through the gel matrix than larger ones.
2.  **(D) Separated DNA fragment stained with ethidium bromide:** After separation, the agarose gel is stained with a fluorescent dye, such as ethidium bromide, which intercalates into the DNA double helix.
3.  **(C) DNA fragments are visualized by exposure to UV radiation:** When the stained gel is exposed to ultraviolet (UV) light, the ethidium bromide fluoresces bright orange, making the separated DNA bands visible.
4.  **(B) DNA bands are cut out from the agarose gel and extracted:** If specific DNA fragments are desired for further use (e.g., in recombinant DNA technology), the visible bands are excised from the gel, and the DNA is purified from the gel slice (a process known as elution).
Therefore, the correct sequence is (A), (D), (C), (B).

Answer

(A), (C), (D), (B)

Answer

(C), (B), (A), (D)

Answer

(A), (D), (C), (B)

Answer

(B), (A), (D), (C)

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